11th International Conference and Expo on Separation Techniques

The blotting technique is a method for identifying biomolecules including DNA, mRNA, and protein at various stages of gene expression. Protein synthesis begins with the expression of a DNA segment, which is then transformed to mRNA and then translated into the desired protein. The target molecule that is sought determines the subtypes of blotting, such as northern, western, and southern. When a DNA sequence is a foundation or code for a protein molecule, the particular DNA molecule of interest can be blotted using the Southern Blotting technique. Northern blotting can be used to identify when DNA is expressed as mRNA for protein creation during gene expression. Finally, the coded mRNA produces the desired protein, which can be identified via Western Blotting. Western blotting (also known as Immunoblotting) is a popular method for detecting proteins and posttranslational modifications on proteins in complicated materials. It uses antibody-based probes to extract particular information a...

  For a variety of reasons, hydrogen has been heralded as the "future fuel." Hydrogen, for starters, has a larger energy yield than the commonly used hydrocarbons. Second, by eliminating the use of exhaustible and polluting fossil fuels, the commercial usage of hydrogen fuel, which produces only water as a byproduct, could assist to address the impending global warming issue. As a result, ongoing research has focused on more efficient and environmentally friendly hydrogen fuel production methods. Hydrogen is a clean fuel source, but purification has been difficult up until recently. Now, a group of scientists has figured out a way to filter hydrogen from other gases by describing a hybrid separation membrane. In view of the present global warming dilemma, the researchers are hopeful that their new membrane will make hydrogen fuel use more efficient and cost-effective. The researchers first created an organic-inorganic hybrid polymeric membrane, which was principally made ...