11th International Conference and Expo on Separation Techniques

The blotting technique is a method for identifying biomolecules including DNA, mRNA, and protein at various stages of gene expression. Protein synthesis begins with the expression of a DNA segment, which is then transformed to mRNA and then translated into the desired protein. The target molecule that is sought determines the subtypes of blotting, such as northern, western, and southern. When a DNA sequence is a foundation or code for a protein molecule, the particular DNA molecule of interest can be blotted using the Southern Blotting technique. Northern blotting can be used to identify when DNA is expressed as mRNA for protein creation during gene expression. Finally, the coded mRNA produces the desired protein, which can be identified via Western Blotting. Western blotting (also known as Immunoblotting) is a popular method for detecting proteins and posttranslational modifications on proteins in complicated materials. It uses antibody-based probes to extract particular information a...

  The branch of analytical chemistry dedicated to the separation, detection, identification, and quantification of biological substances is known as bioanalytical chemistry. It uses chromatography and mass spectroscopy techniques, which are common in analytical chemistry. The purpose of bioanalytical methods is to quantify a drug's and/or its metabolite's or biomarker's concentration in biological fluids such as blood, plasma, serum, urine, and saliva, as well as tissue extracts. However, because biomolecules are designed to work in physiologic circumstances, pH, temperature, and ionic strength changes that occur during many traditional analyses can disrupt molecular interactions and cause structural alterations in proteins and nucleic acids. Existing analytical techniques have been modified to safeguard sensitive biomolecules, and new techniques, such as immunoassay, have been developed to take advantage of biomolecules' specific features. Bioanalytical chemistry...