11th International Conference and Expo on Separation Techniques

The blotting technique is a method for identifying biomolecules including DNA, mRNA, and protein at various stages of gene expression. Protein synthesis begins with the expression of a DNA segment, which is then transformed to mRNA and then translated into the desired protein. The target molecule that is sought determines the subtypes of blotting, such as northern, western, and southern. When a DNA sequence is a foundation or code for a protein molecule, the particular DNA molecule of interest can be blotted using the Southern Blotting technique. Northern blotting can be used to identify when DNA is expressed as mRNA for protein creation during gene expression. Finally, the coded mRNA produces the desired protein, which can be identified via Western Blotting. Western blotting (also known as Immunoblotting) is a popular method for detecting proteins and posttranslational modifications on proteins in complicated materials. It uses antibody-based probes to extract particular information a...

  Environmental chemistry is concerned with the consequences of chemical substances found in nature (air, water, soil, food chains and organisms). Analyticalchemistry is concerned with the development of analytical methods for solving problems in environmental chemistry, such as environmental monitoring, water quality research, and effluent pollutants. Human invention, particularly in the field of analytical chemistry, can also help to solve environmental problems, saving both the environment and populations from harmful health implications. This year's Pittcon will focus on the use of analytical chemistry to environmental and human health issues. Pittcon will address the underlying scientific principles of climate change that affect everyone every day, especially during a global pandemic where health is at the forefront of research efforts and media attention, with a diverse group of researchers, health policy experts, and industry professionals. Characterizing and identifying to...